Which is more suitable for blood glucose detection, sodium fluoride anticoagulant tube or serum separation gel anticoagulant tube?

1, The role of sodium fluoride anticoagulant tube and separation gel anticoagulant tube

The sodium fluoride anticoagulant tube mainly inhibits the enolase in glycolysis through F - to prevent glycolysis, while the separating gel anticoagulant tube uses inert separating gel substances to achieve the goal. Due to its special density, it can be located between serum and blood clots, ensuring that there is no exchange of substances between the two, which can effectively extract the required parts in blood glucose analysis. Although the two mechanisms of action are different, they can both process blood to complete blood glucose testing.

2, Experimental results of sodium fluoride anticoagulant tube and separation gel anticoagulant tube

Although blood glucose testing can be completed, according to the experimental results, the blood glucose indicators measured by the separation gel anticoagulant tube are actually higher than those measured by sodium fluoride. The reason for this is roughly related to the anticoagulant in the sodium fluoride tube. Compared with the separation tube, the anticoagulant agent sprayed in the sodium fluoride tube may have a certain dilution effect on the collected blood, which will affect the measured blood sugar to be lower than the actual result. Therefore, in order to make the blood glucose measured by the sodium fluoride tube closer to the accurate value, the amount of blood sample collected can be increased, which will reduce the impact on diluting the blood.

3, So which is more suitable for blood glucose detection, sodium fluoride anticoagulant tube or separation gel anticoagulant tube?

Due to the different functions of the two, it can be observed in the experiment that there is no change in the behavior of serum or plasma inside the separation tube, while the use of sodium fluoride tube will change the original substances in the serum. Although it can inhibit glycolysis without interference from hemolysis, uric acid, or other drugs, and has relatively high accuracy and precision, small details of deviation cannot be ignored. In practical operation, the method of controlling blood sugar is unstable, and if some original substances in the serum are changed, the results will also be affected. So from this perspective, the separation tube is more suitable for blood glucose detection, but sometimes the appropriate test tube will be selected according to experimental requirements. Generally, it varies from person to person, and flexible selection and use are sufficient.

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